Polymorphism of storage proteins in hexaploid triticale
Sonya Doneva • Hristo Stoyanov
Dobrudzha Agricultural Institute - General Toshevo, 9521, General Toshevo, Bulgaria
Резюме
A collection of 11 triticale (xTriticosecale) varieties created in Dobrudzha
Agricultural Institute (DAI) were analysed using sodium dodecilsuphate
polyacrylamide gel electrophoresis (SDS-PAGE) to describe allelic diversity in
the storage proteins encoded at the Glu-1 (Glu-A1, Glu-B1 and Glu-R1), Glu-3
(Glu-A3 and Glu-B3), Glu-B2 and Gli-R2 loci. Several allels were identified: 7 at
the loci encoding for high molecular weight (HMW) subunits (five for glutenins
and two for secalins), 9 for low molecular weight glutenin subunits and 4 for 75K
γ-secalins. These alleles form 17 allelic configurations. One new allelic form of 75K
γ-secalins was found and it is named ‘new’. Glu-B3 loci was the most polymorphic
with six alleles. Seven varieties posess allel a and six – allel b in Glu-A1 loci.
These alleles are associated with good breadmaking qualities. 73% of triticale
cultivars were homogenous, only 27% showed two ore more diagrams respectively
in HMW glutenin subunits, LMW glutenin subunits and 75K γ-secalins. Thus, the
total number of genotypes in the study increased to 22. These results showed that
triticale varieties grown in DAI don’t exhibit great genetic diversity. Knowledge of
the diversity of the storage proteins will greatly increase our understanding of the influence of glutenins and secalins on the quality parameters of triticale varieties.
