Mycelial compatibility and aggressiveness of Bulgarian Sclerotinia sclerotiorum isolates
Ivan Kiryakov • Keranka Zhecheva
Dobrudzha Agricultural Institute – General Toshevo, 9521, General Toshevo, Bulgaria
Резюме
Mycelial compatibility and aggressiveness of 19 isolates of Sclerotinia
sclerotiorum, originating from common bean and sunflower were studied. The
mycelial compatibility test was carried out on PDA medium with 80 μl/L red food
colorant (Christmas Red – Saye Commerce LTD). The aggressiveness of isolates
was established in greenhouse conditions by inoculation of four plants of GTB
Blyan variety by the straw method for each isolate separately. An AUPDC was
calculated, and isolates were grouped by cluster analysis. Based on their mycelial
compatibility, the studied 19 isolates of S. sclerotiorum were divided into 7 mycelial
compatible groups (MCGs), marked as MCBG1-MCBG7. Regardless of the small
number of samples, the obtained results showed significant genetic variation in
the pathogen population. All MCGs except MCBG7 was built from two or more
isolates. The MCBG7 was made up of isolate, which was incompatible with the other 18 isolates. Based on the AUPDC, the investigated isolates were grouped
into two main clusters, where the second cluster was divided into two subclusters.
Presence of link between the identified MCGs and the isolates aggressiveness was
not established.
Key words:Sclerotinia sclerotiorum, MCGs, Aggressiveness, Common bean,
Sunflower
