1Dobrudzha Agricultural Institute, General Toshevo, Bulgaria
2Technical University - Varna, Bulgaria
1Aksakovo, 9154, Varna region, Bulgaria
Резюме
Nine Aegilops tauschii accessions, D genome donor to Triticum aestivum, are
subjected to this study. Five x-type subunits (null, 1t, 2.1t, 4t, 5t) with slower mobility
and six y-type proteins (10t, 10.1t, 10.2t, 10.3t, 12t, 12.3t) with faster mobility
were identified through SDS-PAGE procedure. They formed seven configurations
of high molecular weight glutenins which were not found in the bread wheat. The
biochemical analysis showed greater amount of low molecular weight glutenins in seeds of the wild progenitor than in common wheat. Six accessions possessed
proteins coded by allele ‘a’, and three - by ‘c’ located in Glu-D3 locus of Triticum
aestivum. А-PAGE electrophoresis resulted in high level of genetic polymorphism
in Gli-Dt1 and Gli-Dt2 loci among the analysed samples. Some proteins of No199
and No219 represented high molecular ω-gliadins with different electrophoretic
mobility than the same proteins in standard wheats. No212, No213 and No227
were distinguished from others through the absence of several slow mobile ω-gliadins
which are typical for bread wheat. This investigation confirmed that the wild
diploid relative Aegilops tauschii seemed as a valuable resource of specific genes
for breeding of high quality grain.
